Generator

Part:BBa_K346010:Design

Designed by: Qianzhu Wu   Group: iGEM10_Peking   (2010-10-18)

Constutitive promoter(BBa_J23108)+RBS+MerR(BBa_K346001)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a copy of BBa_K346001 under the control of a relatively strong, constitutive promoter, BBa_J23108. As the concentration of Hg(II) was fixed, the proportion of Hg-bound MerR dimers was decided by the expression of merR, which is controlled by the different promoter intensities. So when using LacZ as the reporter, we can observe that the colour change with Hg(II) concentration and varied-intensity constitutive promoters.

Source

This part is composed of BioBrick BBa_J23108 and BBa_K346001.

References